Deciphering the myth of cold tolerance in soybean: An overview of molecular breeding applications

The soybean is a source of several dietary components, including milk, protein, and oil. Cold stress has significantly curtailed growth yield in large areas caused high risk to global food security. main objective breeders improve resistance cold stress. Conventional breeding approaches have made significant progress developing tolerance soybean; however, the cost complex genetic mechanism hindered scale these techniques. Molecular tools like quantitative trait loci (QTL), genome-wide association studies (GWAS), transcription factors (TFs), engineering, transcriptome been used identify tolerant genes/QTL develop cultivars Clustered, regularly interspaced short palindromic repeats (CRISPR/Cas9) increase abiotic its use edit genes limited. Mapping QTL accelerated master-assisted selection (MAS) soybean. This review presents detailed overview molecular techniques their cold-tolerant cultivars. Using CRISPR/Cas9 would speed for information will assist researchers uncovering basis adopting most suitable way breed which can thrive under extreme pressure